Quick identification of bacteria by using MALDI-TOF
Matrix Assisted Laser Desorption/Ionisation Time-of-Flight (MALDI-TOF) is a mass spectrometric technique that determines within minutes the identity of a bacterial colony (microorganisms) based on protein patterns. This technique which was investigated for the first time by Het Waterlaboratorium for drinking water research, and is now being used by all Dutch drinking water laboratories as a fast and legally accepted confirmation technique. The analysis time for various microorganisms such as E.coli bacteria, enterococci, Clostridium perfringens, Legionella spp. and L. pneumophila is now reduced to 1-3 days.
Quick analyses results into faster preventive measures by drinking water companies and better protection of the public health, for example in the case of a faecal contamination. Het Waterlaboratorium uses an unique approach because MALDI-TOF can operates as a confirmation technique in routine analysis but also can applied successfully for research purposes. Examples are the source tracing of faecal contamination in extracted water, and the inventories of Aeromoas and coliforms species in the distribution networks of our clients. For more knowledge, see the Knowledge Base
Specialists: Maja Taučer-Kapteijn and Sander Dijkstra