Overview of Publications

Our website is full of examples of research we carry out for various clients. We usually publish the results in reports, memos or presentations. Often these publications are only intended for the client, and therefore are not public. However, we also publish studies in public literature and scientific journals. Below, we provide an overview of the scientific publications that we have published in recent years. Also, collaborations with other international research institutions and universities are provided.


  • Rietjens, I. M. C. M., Schriks, M., Houtman, C. J., Dingemans, M. M. L., & van Wezel, A. P. (2022). Letter to the Editor on Bil et al. 2021 “Risk Assessment of Per- and Polyfluoroalkyl Substance Mixtures: A Relative Potency Factor Approach”. Environmental Toxicology and Chemistry41(1), 7-12.
  • Brekelmans, S., Slootweg, T., Houtman, C.J. 2021. Effect Directed Analysis achterhaalt verantwoordelijke stoffen voor bioassayrespons. H2O-online 7 oktober 2021
  • Corine J. Houtman, Kevin Brewster, Rob ten Broek, Bente Duijve, Yvonne van Oorschot, Martine Rosielle, Marja H. Lamoree, Ruud J.C.A. Steen, Characterisation of (anti-)progestogenic and (anti-)androgenic activities in surface and wastewater using high resolution effectdirected analysis, Environment International, Volume 153, 2021, 
  • Been, F., Pronk, T., Louisse, J., Houtman, C., van der Velden-Slootweg, T., van der Oost, R., Dingemans, M. 2021. Development of a framework to derive effect-based trigger values to interpret CALUX data for drinking water quality. Water Research 193
  • E. Pieke, Onderzoek: Vrijwel geen microplastics in drinkwater, H2O-actueel, 2021


  • TJH Jonkers, M Steenhuis, L Schalkwijk, J Luirink, D Bald, CJ Houtman, J Kool, MH Lamoree, T Hamers. 2020. Development of a high-throughput bioassay for screening of antibiotics in aquatic environmental samples. Science of The Total Environment 729.
  • Houtman CJ, ten Broek R, van Oorschot Y, Kloes D, van der Oost R, Rosielle M, et al. High resolution effect-directed analysis of steroid hormone (ant)agonists in surface and wastewater quality monitoring. Environmental Toxicology and Pharmacology. 2020;80:103460. .
  • Kooijman G, de Kreuk M, Houtman C, van Lier J. Perspectives of coagulation/flocculation for the removal of pharmaceuticals from domestic wastewater: A critical view at experimental procedures. Journal of Water Process Engineering. 2020;34:101161.
  • Zwart, N., Jonker, W., Broek, R.t., de Boer, J., Somsen, G., Kool, J., Hamers, T., Lamoree M.H. and Houtman, C.J. (2020) Identification of mutagenic and endocrine disrupting compounds in surface water and wastewater treatment plant effluents using high-resolution effect-directed analysis. Water Research 168, 115204.


  • L.W. Jonker, K. de Vries, N. Althuisius, D. van Iperen, E. Janssen, R. ten Broek, C.J. Houtman, N. Zwart, T. Hamers, M.H. Lamoree, B. Ooms, J. Hidding, J. Kool, G. Samson. Compound Identification Using Liquid Chromatography and High-Resolution Noncontact Fraction Collection with a Solenoid Valve, SLAS Technology 1-13, (2019):
  • C.J. Houtman, J. Kroesbergen, P.K. Baggelaar,J.H.M. van Lieverloo. Statistical analysis of a large set of semi-quantitative GC–MS screening data to evaluate and prioritize organic contaminants in surface and drinking water of the Netherlands. Science of the Total environment 697 (2019).


  • L. Zlatanovic, A. Knezev, J.P. van der Hoek, J.H.G. Vreeburg. Influence of an Extended Domestic Drinking Water System on the Drinking Water Quality. Water 2018, 10.
  • G.L Ya Zhang, E. van der Mark, A. Knezev,  A. Pinto, B. van den Bogert, W. Liu, W. van der Meer, G. Medema. Assessing the origin of bacteria in tap water and distribution system in an unchlorinated drinking water system by SourceTracker using microbial community fingerprints.  Water Research 138 (2018) 86-96.
  • M. Sousi, G. Liu, S.G. Salinas-Rodriquez, A. Knezev, B. blankert, J. Schippers, W. van der Meer, M.D. Kennedy. Further developing the bacterial growth potential method for ultra-pure drinking water produced by remineralization of reverse osmosis permeate. Water Research 145 (2018) 687-696.
  • S.A. Boers, E.I. Prest, M. Tauèer-Kapteijn, A. Knezev, P.G. Schaap, J.P. Hays, R. Jansen. Monitoring of microbial dynamics in a drinking water distribution system using the culture-free, user-friendly, MYcrobiota platform. 2018.
  • N. Zwart, M.H. Lamoree, C.J. Houtman, J. de Boer, J. Kool, T. Hamers. Development of a luminescent mutagenicity test for high-throughput screening of aquatic samples. Toxicology in Vitro 46, (2018) 350-360.
  • N. Zwart, S.L. Nio, C.J. Houtman, J. de Boer, J. Kool, T.Hamers, M.H. Lamoree. High-Throughput Effect-Directed Analysis Using Downscaled in Vitro Reporter Gene Assays To Identify Endocrine Disruptors in Surface Water. Environmental Science & Technology 2018, 52, 4367-4377.
  • C.J. Houtman (HWL), R. ten Broek (HWL), A. Brouwer (BioDetection systems / Faculty of Earth and Life Sciences).  Steroid hormonal bioactivities, culprit natural and synthetic hormones and other emerging contaminants in waste water measured using  bioassays and UPLC-tQ-MS. Science of the Total Environment 630 (2018), 1492-1501.


  • M. Tauèer-Kapteijn, W. Hoogenboezem, G.J. Medema. Source tracking of E. moraviensis and E. haemoperoxidus. Journal of Water and Health 15 (1)  41-49, 2017.
  • N. Zwart, D. Andringa, W.J. de Leeuw, H. Kojima, M. Iida, C. J. Houtman, J. de Boer, J. Kool, M.H. Lamoree, T. Hamers. Improved androgen specificity of AR-EcoScreen by CRISPR based glucocorticoid receptor knockout. Toxicology in vitro 45, p. 1-9, 2017.
  • C.J. Houtman, J. kroesbergen, P. Behnisch, A. Brouwer, E. Felzel. Bioassays als alternatief voor de chemische monitoring van PCB's en PAK's? H2O-online 31 oktober 2017.
  • N. Zwart, M.H. Lamoree, C.J. Houtman, J. de Boer, J. Kool, T. Hamers, Development of a luminescent mutagenicity test for high-throughput screening of aquatic samples, Toxicology in Vitro, 2017.
  • S. van Nevel, S. Koetzsch, C.R. Proctor, M.D. Besmer, E.I. Prest, J.S. Vrouwenvelder, A. Knezev, N. Boon, F. Hammes. Flow cytometric bacterial cell counts challenge conventional heterotrophic plate counts for routine microbiological drinking water monitoring. Elsevier, Water Research 113 191-206, 2017.
  • C.J. Houtman, R. ten Broek, P. Behnisch, J. Kroesbergen. Investigation of chemical compounds in the water cycle with combinations of bioassays and chemical-analytical techniques. 10th BioDetectors Conference, Sorrento Italy, 6-7 April 2017.


  • B. Martijn, A. v. Rompay, E. Penders, Y. Alharbi, P. Baggelaar, J. Kruithof, I. Rietjens. Development of a 4-NQO TEF approach to enable a preliminary risk assessment of unknown genotoxic compounds detected by the Ames II in UV/H2O2 water treatment samples. Elsevier Chemosphere 144 p338-345, 2016.
  • M.M. Bazri (University of British Columbia), B. Martijn (PWN), J. Kroesbergen (HWL), M. Mohseni (University of British Columbia). Impact of anionic ion exchange resins on NOM fractions: Effect on N-DBPs and C-DBPs precursors, Chemosphere 144, 1988-1995, 2016.
  • A. Knezev (HWL), M. Dignum (Waternet). The ATP measurement and flow-cytometry are better parameters for the assessment of general microbial water quality in drinking water treatment than the HPC. IWA Specialist Conference Microbial Ecology and Water Engineering 2016.
  • M. Taucer-Kapteijn et al. 2016.Screening municipal wastewater effluent and surface water used for drinking water production for the presence of ampicillin and vancomycin resistant enterococci. International Journal of Hygiene and Environmental Health 219, 437–442, 2016.
  • M. Tauèer-Kapteijn, W. Hoogenboezem, G.J. Medema. Environmental growth of the faecal indicator Enterococcus moraviensis. Water Science and Technology: Water Supply 16(4), 971-979, 2016.


  • A. Knezev, H. Smidt, D. van der Kooij. Concentration, identity and behaviour of bacteria predominating in Granular Activated Carbon filters in drinking-water treatment. GACF abstract Iwa biofilm 2015.
  • A. Knezev, Microbial Activity in Granular Activated Carbon filters in drinking-water treatment. PhD thesis, WUR,
  • A. Knezev, S. Strating, B. Lohman. Assessment of the integrity of abstraction system with flowcytometry. Abstract, 2015.
  • A. Knezev, L. Zandvliet, W.A. Oorthuizen, E.J. van der Mark. Value of ATP and Total Cell count for the assessment of general microbial water quality after sand filtration. In Progress in slow sand and alternative biofiltration processes. Pp.51-58. 2014.
  • C.J. Houtman, B.J. Pieters, I. Velzeboer, J. Kroesbergen, Organische stoffen in het Drinkwaterbesluit: 1. Hoe geven we invulling aan de eisen voor 'overige' antropogene stoffen, H2O-Online, 12 oktober 2015
  • C.J. Houtman, I. Velzeboer, T. Slootweg, J. Kroesbergen, Organische stoffen in het Drinkwaterbesluit: 2. Monitoring van 'oude' stoffen, H2O-Online, 12 oktober 2015.
  • A.H. Knol, K. Lekkerkerker-Teunissen, C.J. Houtman, J. Scheideler, A. Ried, J.C. Van Dijk. Conversion of organic micropollutants with limited bromate formation during the Peroxone process in drinking water treatment. Drinking Water Engineering and Science, 8 (2015) 25-34.
  • W. Jonker, M.H. Lamoree, C.J. Houtman, T. Hamers, G.W. Somsen, J. Kool. Rapid activity-directed screening of estrogens by parallel coupling of liquid chromatography with a functional gene reporter assay and mass spectrometry.  Journal of Chromatography A, 1406 (2015) 165-174.



  • M. Tauèer-Kapteijn (HWL), G. Medema (KWR), W. Hoogenboezem (HWL). Comparison between Rapid ID 32 Strep System, MALDI-TOF MS and 16S rRNA gene sequence analysis for the species identification of Enterococcus spp. isolated from water. Water Science & Technology: Water Supply 13 (5), p1383-1389, 2013. 
  • R. Hut, N. van de Giessen, C.J. Houtman. Medicinal Footprint of the population of the Rhine basin. Environmental Research Letters 8(4): 044057 (7p), 2013.
  • M. Schriks, C.J. Houtman, R. van der Oost. The Application of Toxicogenomics for (Drinking) Water Quality Assessment. IWA Publishing. 15 May 2014, ISBN: 9781780406671. 42 pages.
  • W. Jonker, M. Lamoree, C.J. Houtman, J. Kool. Methodologies for Effect-Directed Analysis: Environmental Applications, Food Analysis, and Drug Discovery.  in: Analyzing Biomolecular Interactions by Mass Spectrometry, First Edition, edited by Jeroen Kool and Wilfried M. Niessen. © 2015 Wiley-VCH Verlag GmbH & Co. In press.
  • G. Kooijman, M.L. de Kreuk, C.J. Houtman, J.B. van Lier. The role of colloids in the coagulation/flocculation of pharmaceuticals in domestic wastewater.
  • G. Liu, F.Q. Ling, A. Magic-Knezev, W.T. Liu, J.Q.J.C. Verberk, J.C. van Dijk: Quantification and identification of particle-associated bacteria in unchlorinated drinking water from three treatment plants by cultivation-independent methods. Abstract, Water Research 47, p 3523-3533, 2013.
  • K. Lekkerkerker-Teunissen, A.H. Knol, J.G. Derks, M.B. Heringa, C.J. Houtman, C.H.M. Hofman-Caris, E.F. Beerendonk, A. Reus, J.Q.J.C. Verberk, J.C. van Dijk: Pilot Plant Results with Three Different Types of UV Lamps for Advanced Oxidation. Ozone: Science & Engineering 35:38-48, 2013.


  • Van Lieverloo, Hoogenboezem, Veenendaal en van der Kooij: Variability of invertebrate abundance in drinkingwater distribution systems in the Nethderlands in relation to biostability and sediment volumes. Water Research, 46 p4918-4932, 2012.
  • M. Vital, M. Dignum , A. Magic-Knezev, P. Ross, L. Rietveld,  F. Hammes: Flow cytometry and adenosine tri-phosphate analysis: Alternative possibilities to evaluate major bacteriological changes in drinking water treatment and distribution systems. Water Research 46 p4665-4676, 2012.
  • S.K. Maeng, S.K. Sharma, C.D.T. Abel, A. Magic-Knezev, Kyung-Guen Song, G.L. Amy: Effects of effluent organic matter characteristics on the removal of bulk organic matter and selected pharmaceutically active compounds during managed aquifer recharge: Column study. Journal of Contaminant Hydrology 140–141 p139–149, 2012.
  • R.C.H.M. Hofman-Caris, D.J.H. Harmsen, E.F. Beerendonk, T.H. Knol, C.J. Houtman, D.H. Metz, B.A. Wols: Prediction of advanced oxidation performance in various pilot UV/H2O2 reactor systems with MP- and LP- and DBD-UV lamps. Chemical Engineering Journal 210:520-528, 2012.
  • C.J. Houtman, M. Baneke, T. van der Putten, J. Kroesbergen: Zuiver Water in de Bommelerwaard. In De Snoo GR, Vijver MG, eds, Bestrijdingsmiddelen en waterkwaliteit,  Centrum voor Milieuwetenschappen Leiden; Universiteit Leiden, Leiden, pp 137-149, 2012.
  • K. Lekkerkerker-Teunissen, A.H. Knol, L.P. van Altena, C.J. Houtman, J.Q.J.C. Verberk, J.C. van Dijk: Serial ozone/peroxide/low pressure UV treatment for synergistic and effective organic micropollutant conversion. Separation and Purification Technology 100:22-29, 2012.
  • K. Lekkerkerker-Teunissen, E.T. Chekol, S.K. Maeng, K. Ghebremichael, C.J. Houtman, A.R.D. Verliefde , J.Q.J.C. Verberk, G.L. Amy, R. van Egmond: Pharmaceutical removal during managed aquifer recharge with pretreatment by advanced oxidation. Water Science & Technology: Water Supply 12:755-767, 2012.
  • K. Lekkerkerker-Teunissen, A.H. Knol, P. van 't Hart, G. van Willigen: Ozon en UV-AOP voor effectieve en synergetische omzetting van organische microverontreinigingen. H2O 2012:27-29, 2012.


  • F. Hammes, N. Boon, M. Vital, P. Ross, A. Magic-Knezev, M. Dignum: Bacterial Colonization of Pellet Softening Reactors Used during Drinking Water Treatment. Applied and Environmental Microbiology Feb 2011, p10141-1048, 2011.
  • S.K. Maeng, S.K. Sharma, C.D.T. Abel, A. Magic-Knezev, G.L. Amy: Role of biodegradation in the removal of pharmaceutically active compounds with different bulk organic matter characteristics through managed aquifer recharge: Batch and column studies. water research 45 p4722-4736, 2011.
  • J. Legler, M. van Velzen, P.H. Cenijn, C.J. Houtman, M.H. Lamoree, J.W. Wegener:  Effect-directed analysis of municipal landfill soil reveals novel developmental toxicants in the zebrafish Danio rerio. Environmental Science & Technology 45 p8552-8558, 2011.
  • C.J. Houtman, J. Legler, K.V. Thomas: Effect-Directed Analysis of Endocrine Disruptors in Aquatic Ecosystems. In Brack W, ed, Effect-Directed Analysis of Complex Environmental Contamination, Vol. 15. The Handbook of Environmental Chemistry. Springer Verlag, Berlin-Heidelberg, p237-266, 2011.


  • C.J. Houtman, N.G.F.M. van der Aa, Th. ter Laak: Relatie tussen gebruik geneesmiddelen in Rijnstroomgebied en concentraties in de Rijn. H2O, jaargang 2010, nr. 6, 26 maart 2010.
  • T.L. ter Laak, M. van der Aa, C.J. Houtman, P.G. Stoks, A.P. van Wezel: Relating environmental concentrations of pharmaceuticals to consumption: A mass balance approach for the river Rhine. Environment International 36:403-409, 2010.
  • K. Lekkerkerker-Teunissen , C.J. Houtman, S.K. Maeng, E.  Chekold,  J.Q.J.C.  Verberk, G.  Amy, J. van Dijk: Removal of pharmaceuticals during artificial recharge. Proceedings of the International Symposium on Managed Aquifer Recharge, 2010.
  • M. Tielemans: Zware metalen in drinkwater: balans blijft moeilijk, H2O, 24, 2010.
  • M. Tielemans: Best Practice Guide on the Control of Lead in Drinking Water. Edited bij Colin Hayes, IWA Publising, London, UK, 2010.
  • C.J. Houtman: Emerging contaminants in surface waters and their relevance for the production of drinking water in Europe. Journal of Integrative Environmental Sciences (2010). Vol. 7, issue 4, p. 271-295


  • A. Magic-Knezev: Stand van zaken rond gezondheidsgerelateerde watermicrobiologie. H2O : tijdschrift voor watervoorziening en afvalwaterbehandeling 42 (29). 28-29, 2009.
  • A. Magic-Knezev, B. Wullings, D. van der Kooij: Polaromonas and Hydrogenophaga species are the predominant bacteria cultured from granular activated carbon filters in water treatment. Journal of Applied Microbiology 107 1457–1467, 2009.
  • S. A. Baghoth, M. Dignum, A. Grefte, J. Kroesbergen, G. L. Amy: Characterization of NOM in a drinking water treatment process train with no disinfectant residual. Water Science & Technology: Water Supply—WSTWS | 9.4 | 2009.
  • J. Kroesbergen, K. ten Brinck, T. van der Kaaij: Karakterisering van natuurlijk organisch materiaal,  H2O 30 oktober 2009.
  • A. Magic-Knezev, M.Rosielle, W.Hoogenboezem: The effect of water type on the recovery  of Cryptosporidium oocysts and Gairdia cysts using EnvirochekTM and HemoflowTM pre-concentration techniques. 15th Int. Symposium HRW Microbiology, Greece,2009.


  • C.J. Houtman, S.S. Sterk, M.P.M. van de Heijning, A. Brouwer, R.W. Stephany, B. van der Burg, E. Sonneveld: Detection of anabolic androgenic steroid abuse in doping control using mamalian reporter gene bioassays. Analytica Chimica Acta, 2008.
  • S. K. Maeng, S. K. Sharma, A. Magic-Knezev and G. Amy: Fate of effluent organic matter (EfOM) and natural organic matter (NOM) through riverbank filtration. Water Science & Technology, 2008.


  • G.M. Alink, J.T.K. Quik, E.J.M. Penders, A. Spenkelink, S.G.P. Rotteveel, J.L. Maas, W. Hoogenboezem: Genotoxic effects in Eastern Mudminnow (Umbra pygmaea L.) after exposure to Rhine water , as assessed by use of the SCE and Comet assays: a comparison between 1978 and 2005. Elsevier Mutation Research 631 /RIWA-Rijn 2007.
  • C.J. Houtman, P.E.G. Leonards, W. Kapiteijn, J.F. Bakker, A. Brouwer, M.H. Lamoree, J. Legler, H.J.C. Klamer: Sample preparation method for the ER-CALUX bioassay screening of (xeno-)estrogenic activity in sediment extracts. Elsevier Science of the Total Environment, Vol. 386, 2007.
  • C.J. Houtman, K.M. vd Valk, P. v Bodegom, F. vd Ende, A. Gerritsen, M.H. Lamoree, J. Legler, A. Brouwer: Biomonitoring of estrogenic exposure and identification of responsible compounds in bream from Dutch surface waters. Environmental Toxicology and Chemistry, Vol. 26 no.5 p.898-907, 2007.
  • W. Hoogenboezem, F. Ens en E. Gijsbers: Bijzondere bemonsteringstechnieken in drinkwaterleidingen. H2O, nr 13, 2007.


  • D. van Halem, G. Soppe, J. Kroesbergen, H. van der Jagt: Effectiviteit van met zilver geïmpregneerde keramische potfilters voor waterzuivering op kleine schaal. H2O 25/26, 2006.


  • G. van den Berg, R. van der Plaat, B. Putters, A. Bannink, L. Puijker: MTBE in oppervlaktewater problematisch voor de drinkwatervoorziening?. H2O, 12 p.32-34, 2005.


  • A. Magic-Knezev, D. van der Kooij: Optimisation and significance of ATP analysis for measuring active biomass in granular activated carbon filters used in water treatment. Water Research, 38 p.3971-3979, 2004.
  • A. Magic-Knezev, D. van der Kooij: Molecular analysis of microbial communities in water treatment: biological activated carbon filters. Limnological reports, 35 p.215, 2004.
  • R.J.C.A. Steen, F. Ariese, B. van Hattum, J. Jacobsen, A. Jacobson: Monitoring and evaluation of the environmental degradation rate of the marine antifoulant 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) in a Danish harbour. Chemosphere, 57 p.513-521, 2004.  
  • G.J. Stroomberg, H.Zappey, R.J.C.A. Steen, C.A.M. van Gestel, F. Ariese, N.H. Velthorst, N.M. van Straalen: PAH biotransformation in terrestrial invertebrates - a new phase II metabolite in isopods and springtails. Comparative Biochemistry and Physiology, p.138-137, 2004.
  • P. Diehl, T. Gerke, A. Jeuken, J. Lowis, R. Steen, J. van Steenwijk, P. Stoks, H.G. Willemsen: Early Warning Strategies and Practices Along the River Rhine. Handbook of Environmental Chemistry Vol. 5 The Rhine,Eds. Thomas Knepper, Springer Verlag, Germany, 2004.
  • A. Visser, W. Hijnen, Y. Dullemont, G.J. Medema: Langzame zandfilters als effectieve barriëres voor microorganismen. H2O, 12 p.26-28, 2004.
  • Y. Dullemont, A.Visser, W.Hijnen, G.J. Medema: Eliminatiecapaciteit van langzamezandfiltratie voor micro-organismen bepaald met doseerproeven. H2O, 13 p.22-24, 2004.


  • W.Hoogenboezem, P. Nobel, T. Visser, R. te Welscher: Campylobacter in oppervlaktewater bestemd voor de bereiding van drinkwater. H2O, 19 p.21-23, 2003.
  • H. van Lieverloo, J. Kroesbergen, G. Bakker, W.Hoogenboezem: Systematische beheersing van microbiologische risico's. H2O, 19 p.30-33, 2003.
  • W. Hoogenboezem, E.J.M. Penders: Evaluatie van de genotoxiciteitsgegevens in de Rijn verzameld over de periode 1981 - 2001. RIWA, 2003.
  • J.H.M. Van Lieverloo, D.W. Bosboom, G.L. Bakker, A.J. Brouwer, R. Voogt, J.E.M. de Roos: Sampling and quantifying invertebrates from drinking water distribution mains. Water Research 1 - 12, 2003.
  • E.J.M. Penders, W. Hoogenboezem: Evaluation of the Ames TA98, UMU and Comet assay for quality monitoring of surface water. RIWA, 2003.
  • R. Ghijsen, R. Lindhout, J. Smeenk: De vinger aan de pols. H2O, 25/26 p.30-33, 2003.
  • G.J. Medema, W. Hoogenboezem, A.J. van der Veer: Quantitative risk assessment of Cryptosporidium in surface water treatment. Water Science and Technology, 47  p.241-247, 2003.